Summary of Biochemical Tests

Summary of Biochemical Tests

Tests used to identify Gram Positive Bacteria

• Catalase Test

• Mannitol Salt Agar (MSA)

• Blood Agar Plates (BAP)

• Streak-stab technique

• Taxos P (optochin sensitivity testing)

• Taxos A (bacitracin sensitivity testing)

• CAMP Test

• Bile Esculin Agar

• Nitrate Broth

• Spirit Blue agar

• Starch hydrolysis test

• Motility Agar

• Coagulase Test

Tests used to identify Gram Negative Bacteria

• Oxidase Test

• Sugar (eg glucose) broth with Durham tubes

• Methyl Red / Voges-Proskauer (MR/VP)

• Kliger’s Iron Agar (KIA)

• Nitrate Broth

• Motility Agar

• MacConkey agar

• Simmon’s Citrate Agar

• Urease test

• Sulfur Indole Motility Media (SIM)