The University of Wyoming provides the following as guidelines only for use by IACUC-approved faculty and staff
|
Species |
PO |
SC2 |
IM3 |
IP |
IV (bolus) |
IV (slow)4 |
|
Mouse |
10 µl/g 20-22 gauge Gavage needle |
10 µl/g Scruff, dorsum ≤23 gauge |
Insufficient muscle mass; Not recommended |
20 µl/g ≤21 gauge |
5 µl/g Lateral tail v. 27-30 gauge |
20 µl/g Lateral tail v. 27-30 gauge |
|
Rat |
10 µl/g 18-20 gauge Gavage needle |
10 µl/g Scruff, dorsum ≤20 gauge |
300 µl/site quadriceps m. ≤21 gauge |
20 µl/g ≤21 gauge |
5 µl/g Lateral tail or saphenous v. ≤23 gauge |
20 µl/g lateral tail of saphenous v. ≤23 gauge |
|
Rabbit |
10 ml/kg |
1 ml/kg Scruff, dorsum ≤20 gauge |
0.25 ml/kg Quadriceps m. ≤20 gauge |
5 ml/kg ≤20 gauge |
2 ml/kg Marginal ear v. ≤21 gauge |
10 ml/kg Marginal ear v. ≤21 gauge |
|
Pig |
10 ml/kg |
1 ml/kg Scruff, dorsum ≤18 gauge |
0.25 ml/kg Quadriceps m. ≤18 gauge |
1 ml/kg ≤18 gauge |
2.5 ml/kg Ear v. ≤18 gauge |
5 ml/kg Ear v. ≤18 gauge |
|
Bird |
|
1-3% bdy wt pectoral, interscapular inguinal folds ≤21 gauge |
0.2 ml/100 g Pectoral m. ≤25 gauge |
Not applicable |
|
|
1Does not apply to polyclonal/monoclonal antibody production, or fluid replacement
volumes
2≤3 SC sites per day
3≤2 IM sites per day
45-10 minutes duration